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Cell counting has become an essential method for monitoring the viability and proliferation of cells. A hemacytometer is the standard device used to measure cell numbers in most laboratories which are typically automated to increase throughput. The principle of both manual and automated hemacytometers is to calculate cell numbers with a fixed volume within a set measurement range (105~106 cells/ml). If the cell concentration of the unknown sample is outside the range of the hemacytometer, thedoi:10.21203/rs.3.rs-192770/v1 fatcat:dkynk2t6uffrfebjcep7ryc6zi