Calcium phosphate based biomaterials have received great interest since the main constituent of inorganic mineral component in human bone is hydroxyapatite (HAP). This study aims to extract the natural HAP from bovine femur bone at different calcination temperature with different particle size. The experiment starts with cleaning process of fresh bovine femur bones. Then bovine heated at temperatures of 700°C, 900°C, and 1100°C for three hours. Obtained apatite was characterized using field

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... sion scanning electron microscope (FESEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD). The results indicate that increasing calcination temperature leads to organic free and higher crystallite size of HAP. In terms of particle size, crystallite size is increasing as particle size increases but the increment crystallite size is not significant. The report ring application.