The proteins obtained from separated cell s of neonatal rat dermis , four ce ll populations of epid ermis, a nd a n epiderma l nuclear preparation were ana lyzed by sod ium dodecyl su lfate (SDS) -polyacrylamide gel electrop horesis. Comparison of the results of the inso lubl e proteins of the dermis and epiderm is s how no simil ar ity of the major protein bands, indicating the effective separation of the dermis a nd ep id erm is a nd absence of cross-contam ination. The ge ls of the soluble

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... oteins of t he basal, sp in ous, and granu la r layers of t he ep idermis are very s imilar. Only t he pattern of bands of t he corn ified cells differs in t hat some of these band s are absent and at least three new bands are present. The insoluble proteins have specific differences in the protein content related to t he ce ll structure. An examp le is the nuclear protein bands whi ch correspond with t he most prominent band s in the gels of basal and s pinous l ayer proteins, but are absent, with the possible exception of one band , from gels of cornified cell proteins.