Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Of Proteins Of Newborn Rat Skin I. Cell Strata and Nuclear Proteins

Beverly A. Dale, Irving B. Stern
1975 Journal of Investigative Dermatology  
The proteins obtained from separated cell s of neonatal rat dermis , four ce ll populations of epid ermis, a nd a n epiderma l nuclear preparation were ana lyzed by sod ium dodecyl su lfate (SDS) -polyacrylamide gel electrop horesis. Comparison of the results of the inso lubl e proteins of the dermis and epiderm is s how no simil ar ity of the major protein bands, indicating the effective separation of the dermis a nd ep id erm is a nd absence of cross-contam ination. The ge ls of the soluble
more » ... oteins of t he basal, sp in ous, and granu la r layers of t he ep idermis are very s imilar. Only t he pattern of bands of t he corn ified cells differs in t hat some of these band s are absent and at least three new bands are present. The insoluble proteins have specific differences in the protein content related to t he ce ll structure. An examp le is the nuclear protein bands whi ch correspond with t he most prominent band s in the gels of basal and s pinous l ayer proteins, but are absent, with the possible exception of one band , from gels of cornified cell proteins.
doi:10.1111/1523-1747.ep12598224 pmid:1151113 fatcat:5dkxvawtqnah7bduwp47esgudy