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DNA-free CRISPR-Cas9 gene editing of tetraploid tomatoes using protoplast regeneration
[article]
2021
bioRxiv
pre-print
Wild tomatoes are important genomic resources for tomato research and breeding. Development of a foreign DNA-free CRISPR-Cas delivery system has potential to mitigate public concern about genetically modified organisms. Here, we established a DNA-free protoplast regeneration and CRISPR-Cas9 genome editing system for Solanum peruvianum, an important resource for tomato introgression breeding. We generated mutants for genes involved in small interfering RNAs (siRNA) biogenesis, RNA-DEPENDENT RNA
doi:10.1101/2021.11.02.466947
fatcat:rl3fhlopdfayhnagcz72vbkiyq