Harmonic holography for three-dimensional cellular imaging

Chia-Lung Hsieh, Rachel Grange, Ye Pu, Demetri Psaltis, Bahram Javidi, Jung-Young Son, Manuel Martinez-Corral, Fumio Okano, Wolfgang Osten
2009 Three-Dimensional Imaging, Visualization, and Display 2009  
Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO 3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time and a broad flexibility in the choice of excitation wavelength. We use the coherent SHG signal from
more » ... HG signal from BaTiO 3 nanoparticles for three-dimensional (3D) imaging without scanning. We built a harmonic holographic (H 2 ) microscope which records digital holograms at the second harmonic frequency. Highresolution 3D distributions of these SHG markers in mammalian cells are successfully captured and interpreted by the H 2 microscope.
doi:10.1117/12.820801 fatcat:4xbmzjraljdtpm6gjo22jl4wwq