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Analyzing the gene sequences of terpene synthase (TPS) may contribute to a better understanding of terpenes biosynthesis and evolution of phylogenetic taxonomy. Chamaecyparis formosensis is an endemic and precious conifer of Taiwan. To understand the biosynthesis mechanism of terpenes in this tree, a full length of putative mono-TPS, named as Cf-Pin (GeneBank accession no. EU099434), was obtained by PCR method and RACE extension. The Cf-Pin has an 1887-bp open reading frame and encodes 628<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1515/hf.2009.019">doi:10.1515/hf.2009.019</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/eoqzohz5bngmxggkvhmttccqu4">fatcat:eoqzohz5bngmxggkvhmttccqu4</a> </span>
more »... acids. To identify the function of Cf-Pin, the recombinant protein from Escherichia coli was incubated with geranyl diphosphate, produced one major product, the structure of which was elucidated. GC/MS analysis and matching of retention time and mass spectrum with authentic standards revealed that this product is a-pinene. This is the first report of cloning of a mono-TPS and functionally expressed in E. coli and which could be identified as a-pinene synthase from a Cupressaceae conifer.
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