A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit <a rel="external noopener" href="http://web.nchu.edu.tw/pweb/users/taiwanfir/research/9897.pdf">the original URL</a>. The file type is <code>application/pdf</code>.
Cloning and characterization of α-pinene synthase from Chamaecyparis formosensis Matsum
<span title="2009-01-01">2009</span>
<i title="Walter de Gruyter GmbH">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/ilnjqybbr5gazk2ztezwa7hzva" style="color: black;">Holzforschung</a>
</i>
Preserved Fulltext
Analyzing the gene sequences of terpene synthase (TPS) may contribute to a better understanding of terpenes biosynthesis and evolution of phylogenetic taxonomy. Chamaecyparis formosensis is an endemic and precious conifer of Taiwan. To understand the biosynthesis mechanism of terpenes in this tree, a full length of putative mono-TPS, named as Cf-Pin (GeneBank accession no. EU099434), was obtained by PCR method and RACE extension. The Cf-Pin has an 1887-bp open reading frame and encodes 628
<span class="external-identifiers">
<a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1515/hf.2009.019">doi:10.1515/hf.2009.019</a>
<a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/eoqzohz5bngmxggkvhmttccqu4">fatcat:eoqzohz5bngmxggkvhmttccqu4</a>
</span>
more »
... acids. To identify the function of Cf-Pin, the recombinant protein from Escherichia coli was incubated with geranyl diphosphate, produced one major product, the structure of which was elucidated. GC/MS analysis and matching of retention time and mass spectrum with authentic standards revealed that this product is a-pinene. This is the first report of cloning of a mono-TPS and functionally expressed in E. coli and which could be identified as a-pinene synthase from a Cupressaceae conifer.
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20170811153910/http://web.nchu.edu.tw/pweb/users/taiwanfir/research/9897.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext">
<button class="ui simple right pointing dropdown compact black labeled icon button serp-button">
<i class="icon ia-icon"></i>
Web Archive
[PDF]
<div class="menu fulltext-thumbnail">
<img src="https://blobs.fatcat.wiki/thumbnail/pdf/c3/e5/c3e527395266bd5729683c885325cc051e17163e.180px.jpg" alt="fulltext thumbnail" loading="lazy">
</div>
</button>
</a>
<a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1515/hf.2009.019">
<button class="ui left aligned compact blue labeled icon button serp-button">
<i class="external alternate icon"></i>
degruyter.com
</button>
</a>