Carnobacterium pleistocenium sp. nov., a novel psychrotolerant, facultative anaerobe isolated from permafrost of the Fox Tunnel in Alaska

E. V. Pikuta
2005 International Journal of Systematic and Evolutionary Microbiology  
A novel, psychrotolerant, facultative anaerobe, strain FTR1 T , was isolated from Pleistocene ice from the permafrost tunnel in Fox, Alaska. Gram-positive, motile, rod-shaped cells were observed with sizes 0?6-0?760?9-1?5 mm. Growth occurred within the pH range 6?5-9?5 with optimum growth at pH 7?3-7?5. The temperature range for growth of the novel isolate was 0-28 6C and optimum growth occurred at 24 6C. The novel isolate does not require NaCl; growth was observed between 0 and 5 % NaCl with
more » ... and 5 % NaCl with optimum growth at 0?5 % (w/v). The novel isolate was a catalase-negative chemoorganoheterotroph that used as substrates sugars and some products of proteolysis. The metabolic end products were acetate, ethanol and CO 2 . Strain FTR1 T was sensitive to ampicillin, tetracycline, chloramphenicol, rifampicin, kanamycin and gentamicin. 16S rRNA gene sequence analysis showed 99?8 % similarity between strain FTR1 T and Carnobacterium alterfunditum, but DNA-DNA hybridization between them demonstrated 39±1?5 % relatedness. On the basis of genotypic and phenotypic characteristics, it is proposed that strain FTR1 T (=ATCC BAA-754 T =JCM 12174 T =CIP 108034 T ) be assigned to the novel species Carnobacterium pleistocenium sp. nov. %paper no. ije63384 charlesworth ref: ije53073&
doi:10.1099/ijs.0.63384-0 pmid:15653921 fatcat:q6bhuyup7rh7djws7o42byfkfe