PAI-1 Produced Ex Vivo by Human Adipose Tissue Is Relevant to PAI-1 Blood Level

P. E. Morange, M. C. Alessi, M. Verdier, D. Casanova, G. Magalon, I. Juhan-Vague
1999 Arteriosclerosis, Thrombosis and Vascular Biology  
Human adipose tissue has been shown to produce plasminogen activator inhibitor type 1 (PAI-1). However, the importance of adipose tissue in the regulation of the PAI-1 plasma level is not known. The aim of this study was to investigate the relation between the production of PAI-1 by adipose tissue, plasma PAI-1 level, and variables related to the insulin resistance state. The link between the production of PAI-1 inducers such as tumor necrosis factor-␣ and transforming growth factor-␤ and the
more » ... factor-␤ and the production of PAI-1 by adipose tissue was also evaluated. Blood samples were obtained as soon as possible to the induction of anesthesia from 30 patients undergoing elective abdominoplasty. PAI-1 antigen levels measured in conditioned media after a 19-hour incubation period of adipose tissue explants were significantly correlated with plasma PAI-1 antigen levels (rϭ0.54, Pϭ0.004) and with systemic lipid parameters such as triglycerides and high density lipoprotein cholesterol (rϭ0.46, Pϭ0.014; rϭϪ0.50, Pϭ0.01, respectively) but not with insulinemia and body mass index. PAI-1 production by adipose tissue was correlated with those of TNF-␣ (rϭ0.5, Pϭ0.01) and TGF-␤ (rϭ0.53, Pϭ0.007). These results emphasize the role of adipose tissue in determining plasma levels of PAI-1, with a local contribution of TNF-␣ and TGF-␤ in PAI-1 production by adipose tissue. (Arterioscler Thromb Vasc Biol. 1999;19:1361-1365.)
doi:10.1161/01.atv.19.5.1361 pmid:10323791 fatcat:5umxgwekirdojeo5kcb6igdwq4