Transcriptome Analysis of Genes and Pathways Associated With Metabolism in Scylla Paramamosain Under Different Light Intensities During Indoor Overwintering [post]

Na Li, Junming Zhou, Huan Wang, Changkao Mu, Ce Shi, Lei Liu, Chunlin Wang
2020 unpublished
Background: Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China, Vietnam, and Japan. Genomic and transcriptomic data are scarce for the mud crab. Light intensity is one of the ecological factors that affect S. paramamosain during indoor overwintering. Methods: To understand the energy metabolism mechanism adapted to light intensity, we analyzed the transcriptome of S. paramamosain
more » ... paramamosain hepatopancreas in response to different light intensities (0, 1.43, 40.31 μmol·m-2·s-1). Results: A total of 5052 differentially expressed genes were identified in the LL group (3104 genes were up-regulated and 1948 genes were down-regulated). A total of 7403 differentially expressed genes were identified in the HL group (5262 genes were up-regulated and 2141 genes were down-regulated). Conclusion: The results showed that S. paramamosain adapts to different light intensity environments through the regulation of amino acids, fatty acids, carbon and energy metabolism. Different light intensities had a strong impact on the energy generation of S. paramamosain by influencing oxygen consumption rate, aerobic respiration, glycolysis/gluconeogenesis pathway, the citrate cycle (TCA cycle) and fatty acid degradation. The results of the current study showed that S. paramamosain produced more energy under high light intensities. The findings of the study add to the knowledge of regulatory mechanisms related to S. paramamosain metabolism under different light intensities.
doi:10.21203/ fatcat:rmptcfjp7zhpti5z6d6n5nu5yq