AbstractOxidative stress is a common feature of inflammation-driven cancers, and promotes genomic instability and aggressive tumour phenotypes. It is known that oxidative stress transiently modulates gene expression through the oxidation of transcription factors and associated regulatory proteins. Activated neutrophils produce hypochlorous acid and chloramines that can disrupt DNA methylation via methionine oxidation. The goal of the current study was to determine whether chloramine exposure

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... ults in sequence-specific modifications in DNA methylation that enable long-term alterations in transcriptional output. Proliferating Jurkat T-lymphoma cells were exposed to sublethal doses of glycine chloramine and differential methylation patterns were compared using Illumina EPIC 850K bead chip arrays. There was a substantial genome-wide decrease in methylation four hours after exposure that correlated with altered RNA expression for 24 and 48 hours, indicating sustained impacts on exposed cells. A large proportion of the most significant differentially methylated CpG sites were situated towards chromosomal ends, suggesting that these regions are most susceptible to inhibition of maintenance DNA methylation. This may contribute to epigenetic instability of chromosomal ends in rapidly dividing cells, with potential implications for the regulation of telomere length and cellular longevity.