Purification of a soluble and a wall-bound form of beta-glucosidase from Mucor racemosus

P I Borgia, D W Mehnert
1982 Journal of Bacteriology  
P-Glucosidase activity in crude extracts of Mucor racemosus exists in a soluble form and in a wall-bound form which sediments at 3,500 x g. The soluble form and a wall-bound form were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex chromatography, and SP-Sephadex chromatography. Both forms were identical in all parameters measured. Each enzyme is a glycoprotein of 91,000 daltons, with an identical amino acid composition and Nterminal amino acid of lysine; both contain
more » ... ine; both contain about 10% carbohydrate. Both forms catalyze the hydrolysis of cellobiose and p-nitrophenyl-,3-D-glucoside with identical kinetic constants. on May 8, 2020 by guest
doi:10.1128/jb.149.2.515-522.1982 fatcat:hs3umymaxjeuvbdjnfvahqso3m