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The Effect of High and Low-Intensity Interval Training on TRF1 and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study

Mostafa Khodadoost, Saead Shakeryan, Sareh Arjmand, Masood Nikbakht, Sahid Chamran University of Ahvaz, Sahid Chamran University of Ahvaz, Shahid Beheshti University of Tehran, Sahid Chamran University of Ahvaz
2022 Journal of Rafsanjan University of Medical Sciences  

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and Objectives: The process of chronic diseases and aging is associated with reduced telomere length. The aim of this study was to investigate the effect of high-intensity interval training (HIIT) and low-intensity interval training (LIIT) on telomere repeat binding factor 1 and 2 (TRF1 and TRF2) in Soleus (SOL) muscle as a slow-twitch (ST) and Extensor Digitorum Longus (EDL) muscle as a fast-twitch (FT) of mice skeletal muscle. Materials and Methods: In this experimental study, the subjects
more » ... e C57BL/6 mice (n=24) that were randomly divided into four groups of 6 mice: 1-Base control, 2-control, 3-high-intensity interval training (HIIT), and 4-lowintensity interval training (LIIT). The exercise included 5 days a week for 8 weeks. The gene expression of muscle tissues was measured using RT-PCR. The data were evaluated using two-way ANOVA with Tukey's post hoc test. Results: By comparing ST and FT muscles (p=0.791) and HIIT and LIIT groups (p=0.164) and also the interaction between muscle type and training intensity (p=0.929), there was no significant difference in the expression of TRF1 gene. There was no significant interaction between TRF2 gene expression in ST and FT muscles at different intensities (p=0.868). But the results of Tukey's post hoc test showed that in terms of TRF2 gene expression, HIIT (p=0.031) and LIIT (p=0.035) groups showed more increase compared to the control group. Conclusion: HIIT and LIIT similarly improved the telomere system in skeletal muscle of both muscle types, and this system was more affected by telomere elongation effect of TRF2.
doi:10.52547/jrums.21.1.49 fatcat:faeaaqtfirc6xndy6jbqgcdazu
lang:fa
Citation

Mostafa Khodadoost, Saead Shakeryan, Sareh Arjmand, Masood Nikbakht, Sahid Chamran University of Ahvaz, Sahid Chamran University of Ahvaz, Shahid Beheshti University of Tehran, Sahid Chamran University of Ahvaz. "The Effect of High and Low-Intensity Interval Training on TRF1 and TRF2 Gene Expression in Slow and Fast-Twitch Skeletal Muscles of C57BL/6 Mice: An Experimental Study." Journal of Rafsanjan University of Medical Sciences 21.1 (2022) 49-70