Enrichment culture method, A highly efficient functional bacterium B4 was isolated from polycyclic aromatic hydrocarbon contaminated soil by gradient domestication with pyrene as a single carbon source. The bacterium has strong tolerance to high concentration of polycyclic aromatic hydrocarbons and good degradation ability in a certain concentration range. The degradation rate of pyrene by the strain was 85% under the condition of pyrene concentration of 100 mg/L and inoculation amount of 5%.

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... e degradation effects of the strain on polycyclic aromatic hydrocarbons (PAHs) such as fluorene, naphthalene, fluoranthene and phenanthrene were also investigated. The results showed that the strain B4 had good broad-spectrum degradation performance for PAHs.