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... torate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 19a. NAME OF RESPONSIBLE PERSON a. REPORT b. ABSTRACT c. THIS PAGE 7 19b. TELEPHONE NUMBER (include area code) Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std. Z39.18 The development of these tools has opened up the opportunity for us to begin to characterize tTG generated crosslinks in our cell culture model of crosslink mediated cell motility and invasion. Our current work involves using our tTG enzyme to modify matrix in vitro and measure cellular response (motility and invasion assays, proliferation etc.). These experiments should be completed in 3-5 months. The last goal of this work is to determine if the changes in cell phenotype are mediated through FAK signaling as part of focal adhesions. This work will follow shortly after reproducible crosslingking is achieved in our in vitro model system. KEY RESEARCH ACCOMPLISHMENTS:  Production of recombinant human tissue transglutaminase.  Development of two novel activity assays -one directed at reactive lysines and one toward reactive glutamines.  Development of a assay to determine TG substrate sites in proteins.  Identification of reactive sites on a large number of extracellular matrix proteins including fibronectin.  The development of a database search routine to identify crosslinked peptides from complex mixtures.