and 3β-hidróxi-lanosta-7(8),9,24trieno (agnosterol). Essas substâncias foram identificadas a partir de métodos químicos e físicos, principalmente espectrometria de massas e ressonância magnética nuclear 1D e 2D. A new triterpene, 3β,22ξ,23ξ-trihydroxy-24-methyllanosta-8,24(28)-diene-31-al 22-acetate, named pisosteral, and four known lanostane triterpenes derivatives, 3β,22ξ,23ξ-trihydroxy-24methyllanosta-8,24(28)-diene 22-acetate (pisosterol),

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... e 22-acetate (3-epi-pisosterol), 3β,22ξ,23ξ-trihydroxy-24-methyllanosta-8,24(28)diene 23-acetate and 3β,22ξ,23ξ-trihydroxy-24-ethyllanosta-8,24(28)-diene 22-acetate, were isolated from fruiting bodies of Pisolithus tinctorius, collected in an Eucalyptus plantation. From cultivation of a strain of P. tinctorius isolated from Pinus taeda, four known triterpenes lanostane derivatives, 3β,22ξ-dihydroxy-24-methyllanosta-8,24(28)-diene, 3β,22ξ-dihydroxy-24-ethyllanosta-8,24(28)diene, 3β-hydroxylanosta-8,24-diene (lanosterol) and 3β-hydroxylanosta-7(8),9,24-triene (agnosterol), were isolated. These compounds were identified on the basis of chemical and physical methods, mainly mass spectrometry and 1D and 2D nuclear magnetic resonance.