TOXICITY OF ALKALOID FRACTIONS FROM Psychotria spp. (RUBIACEAE) AGAINST Atta sexdens FOREL, 1908 (HYMENOPTERA: FORMICIDAE)

Dejane Santos Alves, Pilar Canela Garcia Fernandez, Alejandro Martin Martin, Flor Budia, Geraldo Andrade Carvalho, Ronald Zanetti, Denilson Ferreira de Oliveira
2019 Cerne  
TOXICITY OF ALKALOID FRACTIONS FROM Psychotria spp. (RUBIACEAE) AGAINST Atta sexdens FOREL, 1908 (HYMENOPTERA: FORMICIDAE) ALVES, D. S.; FERNANDEZ, P. C.G.; MARTIN, A. M.; BUDIA, F.; CARVALHO, G. A.; ZANETTI, R.; OLIVEIRA, D. F. Toxicity of alkaloid fractions from Psychotria spp. (Rubiaceae) against Atta sexdens Forel, 1908 (Hymenoptera: Formicidae). CERNE, v. 25, n. 2, p.255-262, 2019. HIGHLIGHTS The alkaloid fractions from Psychotria spp. were tested against Atta sexdens. The root fractions
more » ... he root fractions from Psychotria hastisepala presented the highest toxicity against A. sexdens. Branches, stems and leaves fractions of P. hastisepala were also toxic to A. sexdens. Psychotria hastisepala have potential to be employed in the A. sexdens control. ABSTRACT Leaf-cutting ants are the main pests in forest plantations. The most commonly used chemical control for the ants is toxic bait. However, the active ingredients in these baits have been restricted by forest certification organizations, justifying the search for new active compounds to control these insects. Thus, this work aimed to evaluate the formicidal activity of alkaloid fractions from the roots, stems, branches and leaves of Psychotria hastisepala and Psychotria leiocarpa (Rubiaceae) against Atta sexdens Forel, 1908 (Hymenoptera: Formicidae). The alkaloid fractions were obtained from the crude methanolic extracts of P. hastisepala and P. leiocarpa by the acid-base extraction method employing liquid-liquid partitions. The fractions, previously solubilized in propanone, were applied topically to the pronotum of worker ants. Bioassays were conducted in a completely randomized design, with six replicates of each treatment and 10 ants per replicate. Insect survival was assessed daily for 21 days. The median lethal time was estimated by Weibull models. The root fractions from P. hastisepala presented the highest toxicity, followed by those from the branches, stems and leaves. Among fractions from P. hastisepala roots, the most toxic were a fraction that apparently is rich in lipophilic compounds and nonbasic alkaloids and a fraction that is likely rich in quaternary benzophenanthridine alkaloids, protopine alkaloids and tertiary bases. Psychotria leiocarpa fractions were not toxic to this insect. Psychotria hastisepala is potentially useful for the development of new products for A. sexdens control. v.25 n.2 2019 TOXICITY OF ALKALOID FRACTIONS FROM Psychotria spp. (RUBIACEAE) AGAINST Atta sexdens FOREL, 1908 (HYMENOPTERA: FORMICIDAE) 256 CERNE ALVES et al. ALVES et al. TOXICITY OF ALKALOID FRACTIONS FROM Psychotria spp. (RUBIACEAE) AGAINST Atta sexdens FOREL, 1908 (HYMENOPTERA: FORMICIDAE) DAVIS, A. P.; GOVAERTS, R.; BRIDSON, D. M.; RUHSAM, M.; MOAT, J.; BRUMMITT, N. A. A global assessment of distribution, diversity, endemism, and taxonomic effort in the Rubiaceae.
doi:10.1590/01047760201925022632 fatcat:6pp4hno2mjfchhzubvhwebo6t4