Pilot Study: Effect of 3,3′-Diindolylmethane Supplements on Urinary Hormone Metabolites in Postmenopausal Women With a History of Early-Stage Breast Cancer

Kathie M. Dalessandri, Gary L. Firestone, Mark D. Fitch, H. Leon Bradlow, Leonard F. Bjeldanes
2004 Nutrition and Cancer  
Dietary indoles, present in Brassica plants such as cabbage, broccoli, and Brussels sprouts, have been shown to provide potential protection against hormone-dependent cancers. 3,3′-Diindolylmethane (DIM) is under study as one of the main protective indole metabolites. Postmenopausal women aged 50-70 yr from Marin County, California, with a history of early-stage breast cancer, were screened for interest and eligibility in this pilot study on the effect of absorbable DIM (BioResponse-DIM®)
more » ... esponse-DIM®) supplements on urinary hormone metabolites. The treatment group received daily DIM (108 mg DIM/day) supplements for 30 days, and the control group received a placebo capsule daily for 30 days. Urinary metabolite analysis included 2-hydroxyestrone (2-OHE 1 ), 16-= hydroxyestrone (16=-OHE 1 ), DIM, estrone (E 1 ), estradiol (E 2 ), estriol (E 3 ), , and cortisol in the first morning urine sample before intervention and 31 days after intervention. Nineteen women completed the study, for a total of 10 in the treatment group and 9 in the placebo group. DIM-treated subjects, relative to placebo, showed a significant increase in levels of 2-OHE 1 (P = 0.020), DIM (P = 0.045), and cortisol (P = 0.039), and a nonsignificant increase of 47% in the 2-OHE 1 /16 α-OHE 1 ratio from 1.46 to 2.14 (P = 0.059). In this pilot study, DIM increased the 2-hydroxylation of estrogen urinary metabolites.
doi:10.1207/s15327914nc5002_5 pmid:15623462 fatcat:zd5mbuj7wfgdbhkiottrpn6xq4