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Recursive splicing (RS) is a mechanism to excise long introns from messenger RNA precursors. We focused on nuclear RNA, which is enriched for RS splicing intermediates and nascent transcripts, to investigate RS in the mouse brain. We identified new RS sites and discovered that RS is constitutive between excitatory and inhibitory neurons and between sexes in the mouse cerebral cortex. Moreover, we found that the primary sequence context, including the U1 snRNA binding site, the polypyrimidinedoi:10.1101/2020.09.10.291914 fatcat:j46jihbceze4hcd5wvo6z5tf44