Extracellular ATP increases [Ca2+]i in distal tubule cells. II. Activation of a Ca2+-dependent Cl− conductance

Isabelle Rubera, Michel Tauc, Michel Bidet, Catherine Verheecke-Mauze, Guy De Renzis, Chantal Poujeol, Béatrice Cuiller, Philippe Poujeol
2000 AJP - Renal Physiology  
Rubera, Isabelle, Michel Tauc, Michel Bidet, Catherine Verheecke-Mauze, Guy De Renzis, Chantal Poujeol, Bé atrice Cuiller, and Philippe Poujeol. Extracellular ATP increases [Ca 2ϩ ] i in distal tubule cells. II. Activation of a Ca 2ϩ -dependent Cl Ϫ conductance. Am J Physiol Renal Physiol 279: F102-F111, 2000.-We characterized Cl Ϫ conductance activated by extracellular ATP in an immortalized cell line derived from rabbit distal bright convoluted tubule (DC1). 125 I Ϫ efflux experiments showed
more » ... experiments showed that ATP increased 125 I Ϫ loss with an EC 50 ϭ 3 M. Diphenylamine-2-carboxylate (10 Ϫ3 M) and NPPB (10 Ϫ4 M) abolished the 125 Address for reprint requests and other correspondence: P. Poujeol,
doi:10.1152/ajprenal.2000.279.1.f102 pmid:10894792 fatcat:ezpeee5o7zh4xe5xa64wnploza