& aim: Hepatitis B risk factors are a main consideration among jobs related in healthcare. There is a high risk of hepatitis B infection in health care workers, compared to the general population due to higher exposure to patients infected with the virus. The aim of this study was to evaluate the immunity against hepatitis B virus and frequency of HBsAg positive cases among medical staffs of Shahid Beheshti hospital in Yasuj, Iran. Methods: The present cross-sectional, descriptive and

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... study was conducted by interviewing 270 personnel of Shahid Beheshti Hospital of Yasuj in 2016. After completing the questionnaire which included demographic and review vaccination records, 5 ml of blood was taken from the participants. Using an ELISA kit specific antibody levels against hepatitis B virus and HBsAg were measured. The data were analyzed by descriptive statistics and chi-square. Results: A total of 112 patients (41.5%) of the subjects were male and 158 (58.5%) were female. The results indicated that a number of seven subjects (6.2%) had no immunity against hepatitis B virus, 68 (25.1%) had border immunity and 195 (72.2%) had complete immunity. 9 patients (3/3 %) were infected with hepatitis B. Among these participants, 5 patients (55.6%) were female and 4 (44.4%) were male. None of them had any sign of obesity, smoking, high risk sexual behavior, blood transfusion and did not develop jaundice. Conclusion: The present study revealed that the frequency of HBsAg positive cases was in the expected range, and that the samples studied had a favorable safety level.