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Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling
Proceedings of the National Academy of Sciences of the United States of America
The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of its associated guide RNA to one DNA strand, forming an R-loop structure. A dynamic and mechanical description of R-loop formation is needed to understand the biophysics of target searching and develop rational approaches for mitigating off-targetdoi:10.1073/pnas.1913445117 pmid:32123105 fatcat:hoxgqu6zengblkadicgpku4qwi