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Comparative analysis of the standard PCR-Based Replicon Typing (PBRT) with the commercial PBRT-KIT
Plasmids are the main vectors of resistance and virulence genes in Enterobacteriaceae and plasmid typing is essential for the analysis of evolution, epidemiology and spread of antibacterial resistance. The PCR-Based Replicon Typing (PBRT), developed by Carattoli et al. in 2005, was an efficient method for plasmid identification and typing in Enterobacteriaceae. The 2005 PBRT scheme detected 18 replicons in 8 PCR reactions. Recently, the identification of novel replicons and plasmid typesdoi:10.1016/j.plasmid.2017.01.005 pmid:28137396 fatcat:xpgetxfll5dsrfplohxpnxfstm