Crystalline rat liver lactate dehydrogenase was first prepared in this laboratory in 1953 (1) and characterized in some detail (2). A simple graphical method for the calculation of kinetic parameters for two-substrate enzyme systems (3) and a review of the determination of apparent dissociation constants for such systems have been published (4). Recent studies of metal ion status indicate that this enzyme does not contain stoichiometric quantities of heavy metal ion (5). The present report

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... nts the results of mechanism studies and a survey of the specificity requirements for rat liver lactate dehydrogenase. The following is an outline of studies reported in this paper.