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THE EFFECT OF TEMPERATURE OSCILLATION ON INSECT CELL GROWTH AND BACULOVIRUS REPLICATION FOR PRODUCTION OF RECOMBINANT S1 DOMAIN OF THE PORCINE EPIDEMIC DIARRHEA VIRUS SPIKE PROTEIN
Porcine epidemic diarrhea (PED) is an enteric disease in swine caused by an alpha coronavirus. It affects swine of all ages causing acute diarrhea and can lead to severe dehydration and death in suckling piglets. Temperature oscillation can enhance cell viability of sf9 insect cells and baculovirus production of occlusion bodies (OB) and extracellular virus (ECV) compared with constant temperature in stationary culture and suspension culture. The optimal oscillation range was 24 to 28°C. Atfatcat:7bjkfao7yfgc7cbswgpwm7pag4