Investigation of the composition of human milk and assessment of the burden to organic compounds with possible endocrine action [article]

Ελένη Δερμιτζάκη, University Of Thessaly, Εμμανουήλ Τζατζαράκης
The aim of the present study was the qualitative and quantitative analysis of chemicals with potential endocrine action and the determination of the nutrient content of human milk such as fats, carbohydrates, crude and true proteins, total solids and energy. The study was focused on the detection of hexachlorobenzene (HCB), DDTs (op & ppDDE, op & ppDDD, op & ppDDT), parabens (methyl, ethyl, propyl, butyl and benzyl), triclosan, bisphenols A, S and F (BPA, BPS, BPF) in human milk in order to
more » ... mate the dietary exposure of infants during breastfeeding. In total, 49 human milk samples were collected during the first week after birth in the Neonatal Care Unit in the University Hospital of Heraklion, from June 2020 to December 2020. All participants were informed about the study and were asked to sign consent for participation before sample collection. Mothers answered questionnaires about demographic information, dietary and somatometric characteristics, medical history, use of personal care products and information about exposure through other potential environmental sources of the examined compounds. Samples were treated with liquid-liquid extraction and analyzed with gas chromatography and liquid chromatography coupled with mass spectrometry (GC- MS, LC-MS). The mean concentrations for fats were 3.3 g/100 ml (range: 2.0-5.1 g/100 ml), for crude proteins 2.0 g/100 ml (range: 1.2-2.9 g/100 ml), for carbohydrates 7.1 g/100 ml (range: 6.6-7.8 g/100 ml), for total solids 12.5 g/100 ml (range: 11.2-14.2 g/100 ml), for energy 67.9 g/100 ml (range: 55-83 g/100 ml) and for total proteins 1.6 g/100 ml (range: 1.2-2.4 g/100 ml) in 18 tested human milk. Method linearity was acceptable for bisphenols, parabens and triclosan. Limits of detection were ranged from 0.01 ng/ml for propyl paraben and triclosan to 0.32 ng/ml for BPF and recoveries were ranged from 81.5% (BPA) to 121.7% (BPF) except for BPS that was 24.2%. The method accuracy was ranged from 80.0% (benzyl paraben) to 114.2% (triclosan). No sample was detected positive for BPF. BPS was detected in 20% of the samples at median concentration 2.58 ng/ml and BPA in 76% of the samples at median concentration 1.21 ng/ml. Methyl, ethyl, propyl, benzyl and butyl parabens were detected at median concentrations 11.88, 10.18, 2.49, 1.76 and 0.29 ng/ml respectively. For DDTs and HCB, the detection limits were ranged from 0.0005 ng/ml for opDDD to 0.052 ng/ml for ppDDD & opDDT while the quantification limits from 0.002 for opDDE and opDDD to 0.174 ng/ml for ppDDD & opDDT. The average % recovery values were from 58.2% (ppDDD &
doi:10.26253/heal.uth.13328 fatcat:32zeginxhrbvxkrm4jecgmvsgy