Gut Micobiota Alteration by Lactobacillus Rhamnosus Reduces Pro-inflammatory Cytokines and Glucose Level in the Adult Model of Zebrafish [post]

Fatemeh Bootorabi, Farshid Saadat, Reza Falak, Hamed Manouchehri, Reza Changizi, Hasan Mohammadi, Farnaz Safavifar, M.Reza Khorramizadeh
2021 unpublished
Objective: Type 2 diabetes mellitus (T2DM) is still a challenge for physicians to manage patient's circumstances. It is assumed that alterations in the normal flora may be involved in the pathogenesis of T2DM through inducing chronic inflammation. To investigate the effect of Lactobacillus rhamnosus as a common probiotic on T2DM, we induced an experimental model of T2DM in adult male Zebrafish by gradient hyper-glucose accumulation methodology.Results: In this trial three-month old mail adult
more » ... th old mail adult Zebrafish were divided in to four groups including two control groups and T2DM induced groups with or without probiotic treatment. After five days of acclimation, T2DM was induced by a gradient hyper-glucose accumulation methodology. Diabetic fishes had statistically abnormal blood glucose and pro-inflammatory cytokine levels compared to control group (p=0.0001). This results suggest that probiotic intervention hindered the blood glucose level in the T2DM group by decreasing pro-inflammatory cytokines responsible for signaling in T2DM therapeutic modalities.
doi:10.21203/rs.3.rs-505931/v1 fatcat:kzqwdwig6nblpe2zmcsykcyi5y