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CA²⁺-activated potassium channels in smooth muscle cell from cerebral artery of adult rat
Patch clamp methods were used to study the biophysical properties of Ca ²+ -activated potassium (KCa) channels in cerebrovascular smooth muscle cells (CVSMCs) derived from the cerebral arteries of adult rats. Procedures were developed for the enzymatic dissociation of CVSMCs from cerebral arteries. Dissociated cells were maintained at 4°C or cultured at 37°C for 1-3 days prior to use. CVSMCs were identified using a monoclonal antibody specific for smooth muscle a -actin. The calcium-sensitivedoi:10.14288/1.0086586 fatcat:xerpffjdyjeyrgptfvcw3225fa