The fractional rate of cholesterol esterification in very low density lipoprotein-and low density lipoprotein-depleted plasma (FERgoJ was studied in normolipidemic subjects and in individuals with hyperlipidemia and proven coronary artery disease (CAD). The FERnm, was significantly higher than the FER in whole plasma and was significantly higher in normal men than in normal women. In addition, men and women with primary hyperlipidemia had significantly higher FER^m, values relative to their

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... matched controls. The most significant increases in FERHDL values, however, were observed in individuals with CAD. In all patient groups, FERHDL was positively correlated with plasma triglyceride concentration. In addition, FER HDL was negatively related to plasma high density lipoprotein (HDL) cholesterol concentration in all groups except in men with CAD and in normolipidemic women. The gradient gel electrophoretic pattern of HDL from individuals with either low or high FERHD L values indicated an inverse relation between this activity and the relative amount of HDLa, particles. FERHDL likely reflects the metabolic properties of the heterogeneous population of HDL particles in the plasma and may be a function of the relative content of larger and smaller HDL particles. It appears to be a sensitive and reliable functional measure of the particle size distribution in the HDL pool and one of potential clinical value in the assessment of risk for CAD. (Arteriosclerosis and Thrombosis 1991;ll:64-70) (FER). This activity reflects the interaction of LCAT with its endogenous substrates and is usually measured by incubation of plasma, followed by determination of the decrease in concentration of free cholesterol 9 or by the rate of esterification of radioactive unesterified cholesterol. 1011 Changes in FER have been noted in numerous conditions (for a review, see Reference 12), but the findings have been inconsistent. This may have been due to the variety of assay methods employed, the difficulty in evaluating the effect of substrate abnormalities, the presence of potential activators and inhibitors of the enzyme, and changes in the LCAT enzyme mass. Thus, the clinical usefulness of FER has not been accepted. Most of the cholesterol esterification in plasma takes place in the high density lipoprotein (HDL) fraction 2 -13 ; the smaller (HDL3) particles appear to be a preferred substrate, and the larger particles (HDLj), a product of the reaction. 13 -16 Therefore, we decided to estimate the FER in very low density lipoprotein (VLDL)-and low density lipoprotein by guest on July 19, 2018 http://atvb.ahajournals.org/ Downloaded from KEYWORDS • lecithin: cholesterol acyltransferase • high density lipoproteins • coronary artery disease • hyperlipidemia by guest on July 19, 2018 http://atvb.ahajournals.org/ Downloaded from M Dobiasova, J Stribrna, D L Sparks, P H Pritchard and J J Frohlich hyperlipidemia, and coronary artery disease. lipoprotein-depleted plasma. Relation to high density lipoprotein subspecies, sex, Cholesterol esterification rates in very low density lipoprotein-and low density