Pericardial Fluid and Serum Levels of Vascular Endothelial Growth Factor and Endostatin in Patients With or Without Coronary Artery Disease

Jer-Young Liou, Pei-Liang Kuan, Kou-Gi Shyu, Ming-Jen Lu, Hung-Hsin Chao, Bao-Wei Wang
2006 Journal of the Formosan Medical Association  
Background/Purpose: Vascular endothelial growth factor (VEGF) and endostatin are related to ischemic heart disease. This study investigated pericardial fluid and serum levels of VEGF and endostatin in patients with or without ischemic heart disease. Methods: A total of 39 patients (24 patients in the CAD group with significant coronary artery disease; 15 patients in the non-CAD group without coronary artery disease) undergoing open heart surgery were enrolled. In the CAD group, patients were
more » ... p, patients were classified according to good coronary collateralization (Group A; n = 11) or poor coronary collateralization (Group B; n = 13). Pericardial fluid and serum samples were obtained at the time of surgery. VEGF and endostatin were measured by enzyme-linked immunosorbent assay. Results: The levels of endostatin in both serum and pericardial fluid were significantly lower in the CAD group than in the non-CAD group (130.5 ± 37.3 ng/mL vs. 172.4 ± 37.8 ng/mL and 119.0 ± 25.0 ng/mL vs. 143.0 ± 23.5 ng/mL). The concentration of serum VEGF in the CAD group (92.6 ± 18.2 pg/mL) was significantly higher than that in the non-CAD group (75.2 ± 22.3 pg/mL). The concentration of serum VEGF in Group A (100.1 ± 20.7 pg/mL) was significantly higher than that in Group B (84.3 ± 12.4 pg/mL). The levels of pericardial fluid VEGF, serum and pericardial fluid endostatin were not significantly different between Groups A and B. Conclusion: Patients with coronary artery disease have lower serum and pericardial fluid levels of endostatin and higher serum levels of VEGF. Serum level VEGF, but not endostatin, is associated with good or poor collateralization in patients with coronary artery disease. [J Formos Med Assoc 2006;105(5):377-383] The heart is not only a target of neurohormonal activity, but also an active endocrine and paracrine organ. 1 The cardiac tissue produces many physiologically active substances such as cytokines, growth factors and cardiac hormones. 2 These secreted substances may be carried out into the pericardial space. 2 Therefore, analysis of pericardial fluid provides a modality to elucidate the pathophysiology of the heart. Endostatin is a potent endogenous angiogenesis inhibitor and an effective inhibitor of tumor growth in animal models. [3] [4] [5] [6] [7] Endostatin exhibits strong antiangiogenic activity by inhibiting proliferation and migration of endothelial cells, in addition to inducing endo-
doi:10.1016/s0929-6646(09)60133-9 pmid:16638647 fatcat:pmerfa2qufex3iubjx7bmjoixu