The Isolation and Detection of Acanthamoeba in Rural Water Sources of Arak, Iran

M Mosayebi, B Ghorbanzadeh, Z Eslamirad, M Ejtehadifar
2018 unpublished
‫ﻣﺴﯿﺒﯽ‬ ‫ﻣﻬﺪي‬ ‫اﻧﮕﻞ‬ ‫ﮔﺮوه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﻧﮕﻞ‬ ‫اﺳﺘﺎدﯾﺎر‬ ،‫اراك‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﯾﺮان‬ ،‫اراك‬ ‫ﻗﺮﺑﺎن‬ ‫ﺑﻬﺰاد‬ ‫زاده‬ ‫ﻣﺮﺑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﻧﮕﻞ‬ ‫ﮔﺮوه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﻧﮕﻞ‬ ،‫اراك‬ ،‫اراك‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫اﯾﺮان‬ ‫راد‬ ‫اﺳﻼﻣﯽ‬ ‫زﻫﺮا‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﻧﮕﻞ‬ ‫اﺳﺘﺎدﯾﺎر‬ ، ‫اﻧﮕﻞ‬ ‫ﮔﺮوه‬ ،‫اراك‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﯾﺮان‬ ،‫اراك‬ ‫ﻓﺮ‬ ‫اﺟﺘﻬﺎدي‬ ‫ﻣﺼﻄﻔﯽ‬ ‫ﮔﺮوه‬ ،‫آزﻣﺎﯾﺸﮕﺎﻫﯽ‬ ‫ﻋﻠﻮم‬ ‫ﮐﺎرﺷﻨﺎﺳﯽ‬ ‫داﻧﺸﺠﻮي‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬
more » ... ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫آزﻣﺎﯾﺸﮕﺎﻫﯽ‬ ‫ﻋﻠﻮم‬ ‫اﯾﺮان‬ ،‫اراك‬ ،‫اراك‬ ‫راﺳﺘﺎد‬ ‫ﺑﻬﺮام‬ ‫داﻧﺸﺠﻮي‬ ‫ﮔﺮوه‬ ،‫آزﻣﺎﯾﺸﮕﺎﻫﯽ‬ ‫ﻋﻠﻮم‬ ‫ﮐﺎرﺷﻨﺎﺳﯽ‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫آزﻣﺎﯾﺸﮕﺎﻫﯽ‬ ‫ﻋﻠﻮم‬ ‫اﯾﺮان‬ ،‫اراك‬ ،‫اراك‬ :‫ﻣﺴﺌﻮل‬ ‫ﻧﻮﯾﺴﻨﺪه‬ ‫ﻣﺴﯿﺒﯽ‬ ‫ﻣﻬﺪي‬ ‫اﻟﮑﺘﺮوﻧﯿﮏ‬ ‫ﭘﺴﺖ‬ : m.mosayebi@arakmu.ac.ir :‫ﺗﻠﻔﻦ‬ 09108060321 :‫آدرس‬ ‫ﭘﺰﺷﮑﯽ‬ ‫ﻋﻠﻮم‬ ‫داﻧﺸﮕﺎه‬ ،‫ﭘﺰﺷﮑﯽ‬ ‫ﺷﻨﺎﺳﯽ‬ ‫اﻧﮕﻞ‬ ‫ﮔﺮوه‬ ‫اﯾﺮان‬ ،‫اراك‬ ،‫اراك‬ :‫درﯾﺎﻓﺖ‬ 5 / 12 / 91 :‫ﭘﺎﯾﺎﻧﯽ‬ ‫وﯾﺮاﯾﺶ‬ 28 / 1 / 92 :‫ﭘﺬﯾﺮش‬ 1 / 2 / 92 ‫ﭼﮑﯿﺪه‬ :‫ﻫﺪف‬ ‫و‬ ‫زﻣﯿﻨﻪ‬ ‫ﮔﻮﻧﻪ‬ ‫آﻣﯿﺐ‬ ‫دﺳﺘﻪ‬ ‫از‬ ‫آﮐﺎﻧﺘﺎﻣﺒﺎ‬ ‫ﻫﺎي‬ ‫زي‬ ‫آزاد‬ ‫ﻫﺎي‬ ‫ﻫﺴﺘﻨﺪ‬ ‫ﮐﻪ‬ ‫ﺗﻮان‬ ‫ﻣﯽ‬ ‫از‬ ‫ﻣﺤﯿﻂ‬ ‫ﺗﻤﺎﻣﯽ‬ ‫ﮔﻮﻧﻪ‬ .‫ﮐﺮد‬ ‫ﺟﺪا‬ ‫ﻫﺎ‬ ‫ﻣﯽ‬ ‫آﮐﺎﻧﺘﺎﻣﺒﺎ‬ ‫ﻣﺨﺘﻠﻒ‬ ‫ﻫﺎي‬ ‫ﺑﯿﻤﺎري‬ ‫ﺗﻮاﻧﻨﺪ‬ ‫را‬ ‫ﻣﺨﺘﻠﻔﯽ‬ ‫ﻫﺎي‬ ‫ﺑﯿﻤﺎري‬ ‫اﯾﻦ‬ ‫ﺟﻤﻠﻪ‬ ‫از‬ .‫ﮐﻨﻨﺪ‬ ‫اﯾﺠﺎد‬ ‫اﯾﻤﻨﯽ‬ ‫ﺳﯿﺴﺘﻢ‬ ‫ﻧﻘﺺ‬ ‫داراي‬ ‫ﯾﺎ‬ ‫ﺳﺎﻟﻢ‬ ‫دراﻓﺮاد‬ ‫ﻣﯽ‬ ‫ﻫﺎ‬ ‫ﺗﻮان‬ ‫زﺧﻢ‬ ،‫آﮐﺎﻧﺘﺎﻣﺒﺎﯾﯽ‬ ‫ﮐﺮاﺗﯿﺖ‬ ،‫آﻣﯿﺒﯽ‬ ‫ﮔﺮاﻧﻮﻟﻮﻣﺎﺗﻮز‬ ‫اﻧﺴﻔﺎﻟﯿﺖ‬ ‫وﻋﻔﻮﻧﺖ‬ ‫ﭘﻮﺳﺘﯽ‬ ‫ﻫﺎي‬ ‫ﻧﺎزو‬ ‫ﻫﺎي‬ ‫ﻣ‬ ‫در‬ ‫آﮐﺎﻧﺘﺎﻣﺒﺎ‬ ‫آﻣﯿﺐ‬ ‫ﺷﯿﻮع‬ ‫ﺑﺮرﺳﯽ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬ ‫از‬ ‫ﻫﺪف‬ .‫ﺑﺮد‬ ‫ﻧﺎم‬ ‫را‬ ‫ﻓﺎرﻧﺸﯿﺎل‬ ‫آب‬ ‫ﻨﺎﺑﻊ‬ ‫ﺑﺨﺶ‬ ‫روﺳﺘﺎﻫﺎي‬ ‫ﺑﻮد‬ ‫ﻣﺮﮐﺰي‬ ‫اﺳﺘﺎن‬ ‫ﻣﺮﮐﺰي‬. :‫ﺑﺮرﺳﯽ‬ ‫روش‬ ‫ﻣﻘﻄﻌﯽ‬ ‫ﺗﺠﺮﺑﯽ‬ ‫ﻣﻄﺎﻟﻌﻪ‬ ‫اﯾﻦ‬ ‫در‬ 54 ‫از‬ ‫آب‬ ‫ﻧﻤﻮﻧﻪ‬ 36 ‫در‬ ‫اراك‬ ‫روﺳﺘﺎي‬ ‫ﻧﻤﻮﻧﻪ‬ .‫ﺷﺪ‬ ‫ﺗﻬﯿﻪ‬ ‫ﻣﺮﮐﺰي‬ ‫اﺳﺘﺎن‬ ‫واﺗﻤﻦ‬ ‫ﻓﯿﻠﺘﺮ‬ ‫ﮐﺎﻏﺬ‬ ‫ﺗﻮﺳﻂ‬ ‫آزﻣﺎﯾﺸﮕﺎه‬ ‫در‬ ‫ﻫﺎ‬ 42 .‫ﺷﺪ‬ ‫ﻓﯿﻠﺘﺮ‬ ‫ﺳﭙﺲ‬ ‫ﻣﺤﻠﻮل‬ ‫در‬ ‫ﺷﺪه‬ ‫ﻓﯿﻠﺘﺮ‬ ‫ﮐﺎﻏﺬ‬ page saline ‫و‬ ‫ﻣﺨﻠﻮط‬ 70 / ‫آزادزي‬ ‫آﻣﯿﺐ‬ ‫ﺗﺸﺨﯿﺺ‬ ‫و‬ ‫ﺟﺪاﺳﺎزي‬. .. ‫ﻫﻔﺘﻢ)ﺷﻤﺎره‬ ‫آزﻣﺎﯾﺸﮕﺎﻫﯽ،دوره‬ ‫ﻋﻠﻮم‬ ‫ﻣﺠﻠﻪ‬ 4 ‫زﻣﺴﺘﺎن‬ (92 Abstract Background and Objective: Acanthamoeba species are free-living protozoa that can be isolated from all environments. They can bring about different diseases in healthy individuals and immune suppressed patients, for example, Granulomatous Amoebic Encephalitis (GAE), Acanthamoeba Keratitis (AK), Cutaneous and Nasopharyngeal infections. The aim of this study was to evaluate the Acanthamoeba prevalence in rural water sources of Markazi province. Material and Methods: In this cross sectional study, 54 water samples were collected from 36 villages of Markus province. First, the Samples were filtered by filter paper (watchman 42). Next, the filtered paper were placed in page saline solution and centrifuged. Then, the obtained sediment was cultured on non-nutrient agar (NNA) plates overlaid with heat-killed Escherichia coli. After that, the provided smear (after 4-7 days) stained with Geimsa. Results: The samples were positive (33; 61.11%) and negative (21; 38.89%) for Acanthamoeba cyst. The best result for isolation of Acanthamoeba cysts was obtained after shaking of filter paper. Conclusion: A high percentage of rural water sources have been contaminated with Acanthamoeba, which can be the major factor in causing human infections. Therefore, some effective methods are required to prevent from water sources contamination.
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