A Genetic Quality Testing System for Early Stage Embryos in the Mouse

Hideki KATOH, Koji ODA, Kyoji HIOKI, Kaori MUGURUMA
2003 Experimental animals  
We have established a genetic quality testing system for early stage embryos of the mouse. A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 µg/ml) developed by the authors. We demonstrated that two 8-cell embryos of an inbred strain provide sufficient volumes of template DNA for PCR to identify the strain of embryos using four microsatellite markers (D3Mit54, D5Mit18, D6Mit15
more » ... D5Mit18, D6Mit15 and D8Mit50) differentiating 13 inbred strains of mice. This system will be useful in embryo banks that have recently been established worldwide for demonstrating the genetic accuracy of a given strain prior to recovery of live animals.
doi:10.1538/expanim.52.397 pmid:14625405 fatcat:lvhw6tvbrvg35e55rxp5utqx2q