The aim of this research was to obtain a bag log size of white mushroom (Pleurotus ostreatus) capable of producing optimal fruit body with a relatively short time and high Biological Efficiency (BE) value. The study was conducted from February to October 2017. The design of the study used a Completely Randomized Design with two factors. The first factor is the mushroom spawn method (M) consisting of 2 (two) levels which include: m1 = Direct Eksplan Planting (DEP); and m2 = Pure Mycelium Culture

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... (PMC). The second factor is the weight of the substrate (B) consisting of 4 (four) levels which include: b1 = 1.0 Kg; b2 = 1.5 Kg; b3 = 2.0 Kg; b4 = 2.5 Kg. The conclusion (1). The pattern of growth of white oyster mushroom mycelium is best to use the seeds of DEP method with weight of 1 Kg Substrate in 5 weeks long mycelium able to cover bag-log. (2). Production of white oyster mushroom is best using a combination of spawning method of DEP or PMC with a weight of 1.0 kg substrate indicated by Biological Efficiency value of 63.1%.