GATA simple sequence repeats function as enhancer blocker boundaries

Ram P. Kumar, Jaya Krishnan, Narendra Pratap Singh, Lalji Singh, Rakesh K. Mishra
2013 Nature Communications  
Simple sequence repeats (SSRs) account for B3% of the human genome, but their functional significance still remains unclear. One of the prominent SSRs the GATA tetranucleotide repeat has preferentially accumulated in complex organisms. GATA repeats are particularly enriched on the human Y chromosome, and their non-random distribution and exclusive association with genes expressed during early development indicate their role in coordinated gene regulation. Here we show that GATA repeats have
more » ... TA repeats have enhancer blocker activity in Drosophila and human cells. This enhancer blocker activity is seen in transgenic as well as native context of the enhancers at various developmental stages. These findings ascribe functional significance to SSRs and offer an explanation as to why SSRs, especially GATA, may have accumulated in complex organisms. equally and carried out all the experiments, N.P.S. helped in RNA in situ hybridization experiments, L.S. contributed to the idea at early stages and R.K.M. contributed to the planning and analysis. Additional information Supplementary Information accompanies this paper at http://www.nature.com/ naturecommunications Competing financial interests: The authors declare no competing financial interests. Reprints and permission information is available online at http://npg.nature.com/ reprintsandpermissions/ How to cite this article: Kumar, R. P. et al. GATA simple sequence repeats function as enhancer blocker boundaries.
doi:10.1038/ncomms2872 pmid:23673629 fatcat:4lgv7lsfvncklg3cattmetev6a