Determination of LD 50 of Naringenin for its effects on diabetic nephropathy in rats-A pilot study

Mathi Selvam, Murugaiyah Kaliyaperumal
2015 Journal of Chemical and Pharmaceutical Research   unpublished
Diabetic nephropathy is a combined effect of various genetic and environmental factors. Prolonged duration of diabetes, poor glycaemic control and hypertension are major risk factors for diabetic nephropathy. Naringenin, is a biphenolic compound belonging the naturally occurring flavanone, it have been investigated including anticarcinogenic, antimutagenic, anti-inflammatory and antioxidant. However, to our knowledge no reports have recorded the precise biological action of Naringenin against
more » ... diabetic nephropathy in rats. Therefore, the present study was designed with an aim to determine the LD 50 of naringenin for its effects on diabetic nephropathy in rats by the method of Miller and Tainter. The LD 50 value of Naringenin was calculated by both arithmetically and graphically according to the method of Ghosh. Study shows LD 50 value of Naringenin, the 0 % mortality was found to be 100 mg/ kg bwt and also the 100 % mortality was found in the dose of 600mg/kg bwt. After calculating the LD 50 values for the Naringenin, we have then fixed 1/10 th of the LD 50 values of Naringenin as pharmacological doses. We have concluded that Naringenin administered at a dose of 50mg/kg bwt were effective than the rest of the doses particularly when given orally to experimental animals.