Satureja khuzestanica Jamzad is an endemic medicinal plant that grows wild in Iran. Essential oil composition and antimicrobial activity of S. khuzistanica was investigated. The essential oils was obtained by hydro distillation using Clevenger and analyzed by gas chromatography/mass spectrometry )GC/MS(. Forty-four components were identified in S. khuzistanica essential oil. The total represented 99.98% of the oil that major components were identified, carvacrol )92.16%(. As study of

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... al effects of the essential oil of S. khuzistanica was against one Gram-negative bacteria )Escherichia coli( and yeast )Candida albicans( and three Gram-positive bacteria )Staphylococcus aureus, Bacillus cereus, Staphylococcus epidermidis(, in a test based on randomized complete design )RCD( factor with three replication at two method, micro-broth dilution and disc diffusion method. Results showed the highest antimicrobial activities of essential oil of S. khuzistanica on microorganism's was investigated. Antimicrobial activity of S. khuzistanica essential oil on C.albicans yeast more than B. cereus bacteria and it was less than S. epidermidis, E. coli bacterias. The inhibition zones values for bacterial strains, which the essential oils of S. khuzestanica were sensitive in the range of 6.34-54.4 mm and 0.097-0.390 mg/ml, showed effective the oil of S. khuzistanica on Gram-positive bacteria and yeast instead Gram-negative bacteria.