Aim. In order to study rubella virus strain RA-27/3 genetic stability, used for the vaccines production, a molecular genetic study was conducted. Materials and methods. In the study different series of master and work seed of RA-27/3 rubella virus strain by «Microgen», a few lots of rubella vaccines by the different manufacturers, as well as strain «Orlov» of rubella virus were used. RT-PCR followed by restriction, sequencing were performed . Results. Full-genomic sequences of the rubella virus

more »

... strain RA-27/3 by «Microgen», were obtained and presented to GenBank. The full structure correspondence of RA-27/3 rubella virus strain by «Microgen» to the similar rubella strains used by GSK and Merck & Co Inc. has been shown. The RT-PCR method with the subsequent restriction was fulfilled using only domestic reagents. The developed method has been demonstrated as applicable for the identification of the RA-27/3 rubella virus strain as in monopreparation as well as in the combined vaccine preparation. Conclusion. The data obtained make it possible to suggest application of the molecular genetic methods for the vaccine virus identification not only at the production stages, but also in the finished vaccine lots.