De novo assembly is a widely used methodology in bioinformatics. However, the conventional short-read based de novo assembly is incapable of reliably reconstructing the largescale structures of human genomes. Recently, a novel optical label based technology has enabled reliable large-scale de novo assembly. Despite its advantage in large-scale genome analysis, this new technology requires a more computationally intensive alignment algorithm than its conventional counterpart. For example, the

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... -time of reconstructing a human genome is on the order of 10, 000 hours on a sequential CPU. Therefore, in order to practically apply this new technology in genome research, accelerated approaches are desirable. In this paper, we present three different accelerated approaches, multi-core CPU, GPU and FPGA. Against the sequential software baseline, our multi-core CPU design achieved a 8.4× speedup while the GPU and FPGA designs achieved 13.6× and 115× speedups respectively. We also reveal the insights of the design space exploration of this new assembly algorithm on these three different devices by comparing the results.