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New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure based on the use of Phi29 polymerase produces a huge amount of amplified DNA. However, its major disadvantage is to generate a large number of chimeric sequences which can affect the assembly step. The pre-processdoi:10.1186/s40659-016-0099-y pmid:27605096 pmcid:PMC5015205 fatcat:pdddtlf4tnbyria2r2f6jrlft4