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Previous work has characterized how walking Drosophila coordinate the movements of individual limbs (DeAngelis et al., 2019) . To understand the circuit basis of this coordination, one must characterize how sensory feedback from each limb affects walking behavior. However, it has remained difficult to manipulate neural activity in individual limbs of freely moving animals. Here, we demonstrate a simple method for optogenetic stimulation with body side-, body segment-, and limb-specificity thatdoi:10.7554/elife.54183.sa2 fatcat:wprri7zuozd2tgonwzr7igbfcu