Several nicotinic and isoniazid derivatives, most of them containing nitro groups were synthesized by our group, and evaluated for their in vitro antibacterial activity against Mycobacterium tuberculosis H 37 Rv using the Alamar Blue susceptibility test and the activity expressed as the minimum inhibitory concentration (MIC) in mg/mL. The compound 11d exhibited the best result (1.2 µg/mL) when compared with first line drugs such as isoniazid (INH) and rifampicin (RIP). Therefore this class of

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... mpounds could be a good starting point to develop new lead compounds in the treatment of multi-drug resistant tuberculosis. ARKAT USA, Inc. 9a-d; and 11a-d were made directly on the plate. The final drug concentrations tested were 0.01 to 20.0 µL/mL. Plates were covered and sealed with parafilm and incubated at 37 o C for five days. After this time, 25 µL of a freshly prepared 1:1 mixture of Alamar Blue (Accumed International, Westlake Ohio) reagent and 10% tween 80 was added to the plate and incubated for 24 h.