Whole Cell Proteome Regulation by MicroRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach [chapter]

Olivia A. Ebner, Matthias Selbach
2011 Msphere  
Since gene expression is controlled on many different levels in a cell, capturing a comprehensive snapshot of all regulatory processes is a difficult task. One possibility to monitor effective changes within a cell is to directly quantify changes in protein synthesis which reflects the accumulative impact of regulatory mechanisms on gene expression. Pulsed stable isotope labelling by amino acids in cell culture (pSILAC) has been shown to be a viable method to investigate de novo protein
more » ... ovo protein synthesis on a proteome-wide scale (1, 2). One application of pSILAC is to study the regulation of protein expression by microRNAs. Here, we describe how pSILAC in conjunction with shotgun mass spectrometry can assess differences in the protein profile between cells transfected with a microRNA and nontransfected cells.
doi:10.1007/978-1-61779-046-1_20 pmid:21528462 fatcat:dlgwoyxiingkbnsm6yuau6rua4