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Figure 1. Plasmid maps. Representative plasmid maps (A) with 6×HIS and V5 epitope tag and (B) without tags are shown. Restriction sites used in the construction of the vectors or unique restriction sites in multiple cloning sites are shown. Sites in parentheses were destroyed during construction. Bold restriction sites in the polylinker are available for cloning in all auxotrophic versions of the vector; others are found in at least one auxotrophic marker.doi:10.2144/05383bm06 pmid:15786805 fatcat:g4bh6dfsordnlgjwdteciqj6pi