Isolation and Characterization of Phylloplane Associated Bacteria and its in vitro Antagonistic Activity against Bipolaris oryzae

R. Karan, D. Kalaimathi, P. Renganathan, P. Balabaskar
2022 Agricultural Science Digest - A Research Journal  
Rice brown leaf spot is a major fungal disease caused by Bipolaris oryzae. Biological management of brown leaf spot can be achieved by bacteria associated with phyllosphere region of rice crop. Methods: Antagonistic bacteria were isolated from phylloplane of the rice crop using dilution method. Phylloplane bacteria were morphologically and biochemically characterized. Antagonistic activity of bacteria was tested against Bipolaris oryzae using Dual culture technique. Result: Among the isolates,
more » ... B1, PB3, PB6, PB7, PB9 were identified as Pseudomonas fluorescens and PB2, PB4, PB5, PB8, PB10 were identified as Bacillus subtilis by morphological and biochemical characterization. Phylloplane bacteria PB5 showed the maximum inhibition of B. oryzae mycelium (70.00%) followed by PB1 (67.77%). Minimum inhibition of mycelial growth (31.11) was found in PB9.
doi:10.18805/ag.d-5452 fatcat:lusquxiutbbirowblskbj7ednu