The aim of this study was to determine the antimicrobial susceptibilities of Staphylococcus aureus strains isolated from blood cultures of hospitalized patients in the year 2012, retrospectively. Materials and Methods: Antibiotic resistance rates of S. aureus strains isolated in 2012 were analyzed retrospectively. This study involves 58 S. aureus strains isolated from hospitalised patients' blood cultures in intensive care units and clinics. Blood samples were taken to BacT/ALERT FA plus

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... bottles (bioMerieux, France) and these were processed by automatized BacT/ALERT 3D blood culture system (bioMerieux, France). S. aureus strains isolated from blood cultures were identified by VITEC 2 ID-AST Compact System (Biomerieux, France) and the results were evaluated according to the standards of Clinical and Laboratory Standards Institute (CLSI). Results: Of the 58 S. aureus strains, 27 (46.6 %) were methicillin susceptible S. aureus (MSSA) and 31 (53.4%) were methicillin resistant S. aureus (MRSA). All strains were found susceptible to vancomycin, teicoplanin and linezolid. Compared to MSSA MRSA isolates were more resistant to antibiotics and these differences were found statistically significant. Conclusion: The determination of agents causing infection and antibiotic resistance profile for all centers is important in order to guide empirical treatment and in the establishment of antibiotic usage guidelines.