The biting midges (Ceratopogonidae) are relatively well studied dipteran family that includes over 6000 extant species in 110 genera. They are known as vectors of several arboviruses of veterinary importance including bluetongue, bovine ephemeral fever, African horse sickness and Akabane virus, as well as the medically important Oropouche virus. A key For Ceratopogonidae of Markazi province is presented. Four genera, 8 subgenera and 9 species were found. 5 species which marked with an asterisk (*), are new records for Iran.