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Cultural Estimation of Yeasts on Seaweeds
Five per cent suspensions of freshly harvested seaweeds were used as an inoculum to develop a selective medium for epiphytic yeasts. Conditions for satisfactory yeast growth and visualization as red colonies on membrane ifiters were obtained by supplementing a basal glucose-Trypticase-yeast extract-agar at pH 7.0 with 100 mg each of chloramphenicol and 2,3,5-triphenyl tetrazolium chloride per liter. Maximal counts were obtained by triturating the algae in prechilled (4 C) seawater with adoi:10.1128/am.22.4.507-512.1971 fatcat:fwytoeckuzfjxovwtxnyfipaoa