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Three Dimensional In Vitro Culture of Murine Secondary Follicles in a Defined Synthetic Matrix
Journal of Biomaterials and Nanobiotechnology
Ovarian follicle growth in three dimensional (3D) matrices in vitro has limitations: a) matrices don't expand as follicles grow, b) requirements for enzyme-mediated retrieval, and c) animal-derived components prevent clinical application. Therefore, we evaluated N-Isopropylacrylamide (SFX-1), a novel synthetic 3D culture matrix, for follicle culture. Groups of three murine secondary follicles were encapsulated in 50 µL of DMEM/F12-1%ITS-10%FCS (DMEM/F12) or SFX-1 (3:2 v/v DMEM/F12) or Matrigeldoi:10.4236/jbnb.2018.93014 fatcat:5k6bghw3czbmxobu54q7fyx7oy