The Esterase Activity of Plain and Dextrose Broth Cultures of the Typhoid Bacillus. Studies in Bacterial Metabolism, XXXIX

A. I. Kendall, J. P. Simonds
1914 Journal of Infectious Diseases  
A series of experiments to be reported soon" have shown that both the filtrates of broth cultures of acid-fast bacilli and the organisms themselves contain active lipase. The period of maximum lipase activity of these organisms, furthermore, coincides rather closely in time with the period of maximum vegetative activity of the bacteria. This phenomenon was established by comparing the fat-and estersplitting power of the filtrates of these cultures with their proteolytic activity under the same
more » ... ity under the same conditions of incubation. Bacteria which are not acid-fast also appear able to split fats. According to Rubner," this phenomenon is usually a "fat fermentation," not brought about by a fat-splitting ferment (lipase), but rather by the direct action of the bacterial cytoplasm. Other observers, notably Sommaruga," and Wells and Corper," believe a true lipolysis takes place. Several well-known pathogenic bacteria, according to these observers, possess a lipase. It is not definitely determined whether this ferment is a true endo-lipase liberated only when the organisms are disrupted, ordinarily by autolysis, or whether this lipase is, in part at least, a true exo-ferment. The composition of the medium in which these organisms are grown, furthermore, may conceivably influence the nature and extent of the lipolytic activity of these bacteria, and it was with these several possibilities in view that the following experiments were made.
doi:10.1093/infdis/15.2.354 fatcat:cgdnv22b6bav7glgm5rajzgrvm