Strains of enterotoxigenic Escherichia col that produce 987P fimbriae (987P+ strains) colonize the small intestines and cause diarrhea in neonatal (<6-day-old) pigs but not in weaned pigs. However, 987P+ E. coli strains adhere in vitro to intestinal epithelial cells from pigs of both ages. Two intestinal components, designated 987R and 987M, bind 987P fimbriae (987P) on Western blots (immunoblots). We examined brush borders (BB) and intestinal washes (IW) from pigs to determine if they contain

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... ne if they contain glycolipids which bind 987P. Total glycolipid extracts from BB and IW of 4-week-old pigs were separated on thin-layer chromatograms and overlaid with purified 987P. Bound 987P were detected with 987P-specific antiserum. 987P bound to at least one moiety in both BB and IW glycolipids and also bound to several purified glycolipids, including 4789 Vol. 62, No. 11 on May 5, 2020 by guest http://iai.asm.org/ Downloaded from