Detection of Lymnaea columella infection by Fasciola hepatica through Multiplex-PCR

Kelly Grace Magalhães, Liana Konovaloff Jannotti Passos, Omar dos Santos Carvalho
2004 Memórias do Instituto Oswaldo Cruz  
From complete mitochondrial DNA sequence of Fasciola hepatica available in Genbank, specific primers were designed for a conserved and repetitive region of this trematode. A pair of primers was used for diagnosis of infected Lymnaea columella by F. hepatica during the pre-patent period simultaneously with another pair of primers which amplified the internal transcribed spacer (ITS) region of rDNA from L. columella in a single Multiplex-PCR. The amplification generated a ladder band profile
more » ... r band profile specific for F. hepatica. This profile was observed in positive molluscs at different times of infection, including adult worms from the trematode. The Multiplex-PCR technique showed to be a fast and safe tool for fascioliasis diagnosis, enabling the detection of F. hepatica miracidia in L. columella during the pre-patent period and identification of transmission areas.
doi:10.1590/s0074-02762004000400013 fatcat:hpcuz7prknbl7f2br456nhrtmu